Composite

Part:BBa_K1362107

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-08)

RBS + NpuDnaE C-intein RFC[105] assembly construct with additional N-teminal insertion site

This assembly construct is part of the iGEM team Heidelberg 2014's intein toolbox. It allows for easy generation of translational units of the Npu DnaE C-intein flanked by N and C terminal POIs as described in RFC[105]. This part is very similar to BBa_K1362101 and BBa_K1362105 but provides a secondary Gate for the insertion of a protein or peptide of interest to the N-terminus of the intein. As no splicing occurs on that side of the Intein, the latter will stay with the intein after splicing. BBa_K1362106 is the complementary N-intein part.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 826
    Illegal AgeI site found at 938
    Illegal AgeI site found at 2020
    Illegal AgeI site found at 2132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1115
    Illegal BsaI site found at 2309
    Illegal BsaI.rc site found at 40
    Illegal BsaI.rc site found at 1234


[edit]
Categories
Parameters
None